Phospho-rps6

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August undefined, 2024

WebApr 10, 2024 · Purpose: To present a case of a chemotherapy regimen combining a fibroblast growth factor receptor (FGFR) and mitogen-activated protein kinase kinase … WebRPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD. Distribution Cytosol, nucleus, ER, ubiquitous expression. Function Phosphorylation events at a cluster …

Phospho-S6 (Ser240, Ser244) Polyclonal Antibody (PA5-85822)

Web40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors ... WebMar 9, 2024 · Flow Cytometry - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691) Flow Cytometry analysis of PC-3 (Human prostate adenocarcinoma epithelial … fish tank long island

PE anti-RPS6 Phospho (Ser235/Ser236) Antibody

WebAn immobilized capture antibody specific for Ribosomal Protein S6 / RPS6 binds both phosphorylated and unphosphorylated Ribosomal Protein S6 / RPS6. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format. Complete Your Research WebDot blot analysis of RPS6 (phospho S240 + S244) labeled with ab215214 at 1/1000 dilution. Lane 1: RPS6 (phospho S240 + S244) peptide. Lane 2: RPS6 (phospho S240) peptide. Lane 3: RPS6 (phospho S244) peptide. Lane 4: RPS6 non-phospho peptide. Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution was used as secondary antibody. WebPhospho-S6 (Ser240, Ser244) Antibody (701845) in ICC/IF. For immunofluorescence analysis HEK-293 cells were fixed and permeabilized for detection of RPS6 (pS240/244) using RPS6 (pS240/244) Recombinant Rabbit Monoclonal Antibody ( Product # 701845, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, … candy candy candy land

Phospho-S6 Ribosomal Protein (Ser235/236) Antibody

Purified anti-RPS6 Phospho (Ser244) Antibody - BioLegend

WebWB analysis using 80206-1-RR. Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80206-1-RR (Phospho-S6 Ribosomal protein (Ser236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. WebRPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD. Distribution Cytosol, nucleus, ER, ubiquitous expression. Function Phosphorylation events at a cluster … candy candy buffet for baby showerWebResults: The activity of a downstream effector of the HER2 pathway phosphorylated ribosomal protein S6 (p-rpS6), was suppressed by trastuzumab in the parental cell lines … fish tank low ph

"WebProduct name Anti-RPS6 (phospho S235) antibody [EP1338 (2)Y] - Ribosome Marker See all RPS6 primary antibodies Description Rabbit monoclonal [EP1338 (2)Y] to RPS6 (phospho S235) - Ribosome Marker Host species Rabbit Tested applications Suitable for: WB, IP, IHC-P, Dot blot more details Unsuitable for: Flow Cyt or ICC/IF Species reactivity " - Phospho-rps6

Phospho-rps6

Recombinant Anti-RPS6 (phospho S235) antibody [EP1338(2)Y

WebPhospho-S6 Ribosomal Protein (Ser235/236) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 … WebMay 3, 2024 · (i) Ribosomal protein S6 (rpS6) is a downstream substrate of PI3K/akt/mTOR/ kinase and MAPK kinase pathways and its dephosphorylation is also triggered by DNA double strand breaks. Phospho-rpS6 is reliably measurable by flow cytometry and thus has the potential to function as a biomarker of responsiveness to several therapeutic agents.

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WebFeb 6, 2024 · We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully... WebMar 9, 2024 · Anti-RPS6 (phospho S235 + S236) antibody [SP50] See all RPS6 primary antibodies Description Rabbit monoclonal [SP50] to RPS6 (phospho S235 + S236) Host species Rabbit Tested applications Suitable for: Dot blot, IHC-P, ICC/IF, Flow Cyt more details Species reactivity Reacts with: Mouse, Human Immunogen

WebAnimals were fasted overnight and refed ad lib for 4 h. Quantification of phospho-rpS6 is shown at the right, normalized to total rpS6. (*) P < 0.05 by Student's t-test. (D) Immunofluorescence staining of phospho-S6 (red) and TFE3 (green) in control (NTC) cells and cells lacking TSC1 or TSC2. Cells were starved or starved and restimulated with ... WebDec 21, 2007 · The level of phosphorylated S6 ribosomal protein expression was predictive of early tumor response to the mTOR inhibitor, suggesting that this is a promising new predictive sarcoma marker for...

WebRayBio® Phospho-RPS6 (S235) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated RPS6 protein in your experimental model system, you can verify pathway activation in your cell lysates. WebRPS6 Rabbit Polyclonal Antibody: 50μl: 593.00元: 加入购物车: AG3129: Phospho-S6 Ribosomal Protein (Ser235/236) Mouse Monoclonal Antibody: 50μl: 998.00元: 加入购物车: AG3133: Phospho-S6 Ribosomal Protein (Ser235/Ser236) Rabbit Monoclonal Antibody: 50μl: 998.00元: 加入购物车: AG3136: Phospho-S6 Ribosomal Protein (Ser240/244 ...

WebCells were then intracellularly stained with 5 µg/mL (1:100 dilution) of Purified anti-RPS6 Phospho (Ser244) Antibody overnight at 4°C, after which proteins were visualized with Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.

WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for … fish tank maconWebRPS6 Antibodies Target Information Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. fish tank made to measureWebImmunohistochemical analysis of Phospho-RPS6 pSer240 in paraffin-embedded human breast carcinoma using Phospho-RPS6 pSer240 polyclonal antibody (Product # PA5-36759) at a dilution of 1:100. Western blot analysis of Phospho-S6 (Ser240) in Lane 1: HeLa whole cell lysate (40 µg), Lane 2: HeLa treated with serum starved (24h) whole cell lysate (40 ... candy candy charactersWebProduct name Anti-RPS6 (phospho S235) antibody [EP1338 (2)Y] - Ribosome Marker See all RPS6 primary antibodies Description Rabbit monoclonal [EP1338 (2)Y] to RPS6 (phospho … fish tank macon gaWebPhospho-S6 Ribosomal Protein (Ser240/244) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serines 240 and 244. This antibody does not detect S6 ribosomal protein phosphorylated … fish tank magnetic cleanerWebabcam抗体：Phospho anti-RPS6 (S235 + S236) antibody (ab12864)货号：ab12864product name：Anti-RPS6 (phospho S235 + S236) antibodyDescription：Rabbit p candy candy clip arthttp://genesdev.cshlp.org/content/19/18/2199.full candy candy creator