H9c2 cell transfection
WebOverexpression of PKCδ induced by high glucose in H9C2 cells impaired GLP-1 post-receptor anti-apoptotic signaling pathways by inhibition of Akt phosphorylation. ... according to the manufacturer’s instruction. Co-transfection of RKCβ siRNA and PKCδ siRNA was established at a proportion of 1:1. Total RNA was isolated from H9C2 cells using ... WebCell transfection. H9C2 cells were plated in 35-mm culture dish at 1.5×10 5 cells/dish for 12 h. The cells were transfected with I, IC, M, MC (50 nM; Shanghai GenePharma Co., Ltd.) using riboFECT™ CP Reagent and buffer (Guangzhou RiboBio Co., Ltd.). RiboFECT™ CP-I/IC mixture and DMEM were added to the cells at 37°C and incubated for 48 h.
H9c2 cell transfection
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WebJul 16, 2024 · Transfection of HSP70 into H9C2 cells could reduce the corresponding effect, increase cell viability and anti-apoptotic signal pathway, and reduce the apoptotic rate and pro-apoptotic signal pathway. After hypoxia and reoxygenation, the expression of STIM1/IP3R and intracellular calcium concentration of HSP70-overexpressed H9C2 … WebView Cell Phone Numbers View Dora's Phone Numbers. Addresses. Dora's Current Address 748 Fawn Creek St Leavenworth, KS 66048 Other Locations 1234 Fawn Creek St , Leavenworth, KS. 1234 Linda Spur , Killeen, TX. 1234 PO Box ...
WebGrowing H9C2 cells and trying to ensure they maintain the phenotye of Cardiac cells. They are grown in T150 flasks, maintained and grown in 25ml of MEM (containing 10% FCS, Na+ Pyruvate,... Weba. Change your culture conditions: use an endotoxin and contaminant free medium for culture and complexes formation. Any trace of endotoxin can …
WebThe reagent allows for transfection to be performed both in presence or absence of serum. The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively … WebRecommended Transfection Reagents. Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 “It has exceeded my expectations and has worked better than our …
WebRecommended Transfection Reagents. Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 “It has exceeded my expectations and has worked better than our current reagent (FuGENE® HD) in H9c2 cells.” – Researcher, USA; RNAi transfection (siRNA/miRNA) and gene silencing: Lipofectamine® RNAiMAX
WebBased on our proteomic analysis, its presence in EVs was linked to hypoxia in HL1 and H9c2 cells, and we hypothesize that EV-associated ENPL may have a cardioprotective role by reducing cardiomyocyte ER stress. ... Transfection of H9c2 and HL1 Cells with pMAXGFP-ENPL Plasmid. H9c2 and HL1 cells were cultured up to 80% confluency in … adolescent dialectical dilemmasWebH9C2 cells showed almost identical hypertrophic responses to those observed in primary cardiomyocytes. This finding validates the importance of H9C2 cells as a model for in vitro studies of cardiac hypertrophy and supports current work with human cardiomyocyte cell lines for prospective molecular studies in heart development and disease. adolescent diagnostic interviewWebApr 13, 2024 · H9c2 cells were pretreated with 32 μm Fer-1 for 24 h before synchronization. Samples in an experiment represented one independent replicate, and every experiment needed to be repeated at least 3 times. Cell Transfection The oligonucleotides were transfected into cells according to the manufacturer’s instructions. jsonhintアノテーション javaWebAdd 1μg of plasmid DNA to the medium, and mix. For a 3:1 ViaFect™ Transfection Reagent:DNA ratio, add 3μl of ViaFect™ Transfection Reagent, and mix immediately. Incubate the ViaFect™ Transfection Reagent:DNA mixture for 5–20 minutes at room temperature. Optional: Add mixture to cells without an incubation period. adolescente agressivoWebMar 2, 2024 · H9c2 cells were lysed in RIPA lysis buffer (Epizyme Biotech, Shangai, China) and centrifuged at 12,000 ... On the other hand, transfection of miR-31-5p mimic in H9c2 cells led to decreased levels of circPan3 compared with the control (Fig. 4G). Together, these results indicated that miR-31-5p is involved in DOX-induced cardiotoxicity via the ... json kml変換 フリーソフトWebTransfection of H9C2 cells with 20 M.O.I of GFP-mCherry-LC3 adenovirus for 24 h and then treated with DOX and rapamycin for 24 h and 8 h respectively. Then remove the culture medium, cold methanol fix for 15 min in 4 ℃. After two times washing by PBS, the nuclei were stained by incubating DAPI with the samples for 5 min at room temperature. ... adolescente che pensaWebH9c2 (2-1) CRL-1446 ™. H9c2 (2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue that exhibits many of the properties of skeletal … adolescent diagnosis