WebMar 29, 2024 · The SMARTer ® RACE 5′/3′ Kit (TaKaRa, Dalian, China) was used to obtain the full-length sequence of lnc001776. The RNA was reverse transcribed to cDNA, as per the directions, and then 5′ and 3′ PCR amplification was carried out, respectively. ... The process of nuclear RNA extraction is the same as cytoplasmic RNA. The successfully ... WebRapid method for the isolation and purification of both nuclear and cytoplasmic RNA from cultured animal cells and animal tissue samples. Catalog No. BP280550. This item has …
Nuclear and cytoplasmic protein extraction kit Sigma-Aldrich
WebThe NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilize and separate cytoplasmic and nuclear proteins into ... WebItem SurePrep Nuclear or Cytoplasmic RNA Purification Kit; Company Fisher BioReagents; Price Supplier Page View Company Product Page; Catalog Number BP280550; Quantity 1 EA Nuclear (25 preps) and Cytoplasmic (50 preps) RNA Purification Kits; Source Cell/Tissue; Nucleic Acid Type Total RNA how many calories per pistachio
Multiple-Analyte Kits Norgen Biotek Corp.
WebApr 13, 2024 · The reactions were performed at 55 °C for 15 minutes to denature the RNA and stopped by adding 310 μL water followed by purification of the conjugated RNA … WebNov 15, 2024 · 3.8 Cytoplasmic and Nuclear RNA Extraction. 1. Add 100 μl ice-cold Cytoplasmic Extraction Reagent I (CER I) to a tube of cells or tissue powder (pellet) (see Note 29). 2. Vortex the tube vigorously for 15 s. 3. Incubate the tube on ice for 10 min. 4. Add 5.5 μl ice-cold Cytoplasmic Extraction Reagent II (CER II) buffer to the tube. 5. WebThe Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. Features of the NE-PER … how many calories per pound of potato